Please enter vector sequence or multiple cloning site sequence

(automatically adjusted to 5 'to 3' direction according to the restriction sites)

Whether to retain restriction sites after recombination: No Yes

Please select restriction sites (must be in 5 'to 3' direction):

Number of inserts: 1 2 3 4 5 6

Please enter each inserted DNA fragment: (The sequence orientation defaults to 5 'to 3')

Insert Fragment 1

Insert Fragment 2

Insert Fragment 3

Insert Fragment 4

Insert Fragment 5

Insert Fragment 6